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p815-gfp  (Thermo Fisher)


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    Thermo Fisher p815-gfp
    P815 Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p815-gfp/pm15336644-283-0-17
    Average 90 stars, based on 1 article reviews
    p815-gfp - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Maximizing antigen targeting to the proteasome for gene-based vaccines.
    Article Snippet: P815-GFP is an H-2d mastocytoma cell line stably transfected with GFP and maintained in 800 Ag/ml geneticin (Invitrogen).

    Transfection:

    Article Title: Maximizing antigen targeting to the proteasome for gene-based vaccines.
    Article Snippet: P815-GFP is an H-2d mastocytoma cell line stably transfected with GFP and maintained in 800 Ag/ml geneticin (Invitrogen).



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    T cells transduced with the dominant-active α-chain TCRs killed cognate tumor cells in vitro. T cells of intact C57BL/6 mice (H-2 b ) were modified with identified dominant-active TCRα from the repertoire of primarily activated effectors (EF) or reactivated memory T cells (EM) (Figs. and ). Transduced T cells were mixed with the allogeneic <t>mastocytoma</t> <t>P815-GFP</t> (K d D d ) at a ratio of 2:1. Nontransduced (NTR) T cells were used as the control. Cytotoxic activity of transduced T cells was analyzed by flow cytometry after 24 h of culturing by evaluating the percentage of dead P815 tumor cells as GFP + PI + cells. Data from 2 to 4 independent experiments are shown as mean ± SD. * P < 0.05, ** P < 0.01 versus the NTR control, unpaired Student’s t test.
    P815 Gfp, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p815-gfp/P815/pmc13082541-42-14-23
    Average 96 stars, based on 1 article reviews
    p815 gfp - by Bioz Stars, 2026-11
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    p815-gfp  (Thermo Fisher)
    90
    Thermo Fisher p815-gfp
    T cells transduced with the dominant-active α-chain TCRs killed cognate tumor cells in vitro. T cells of intact C57BL/6 mice (H-2 b ) were modified with identified dominant-active TCRα from the repertoire of primarily activated effectors (EF) or reactivated memory T cells (EM) (Figs. and ). Transduced T cells were mixed with the allogeneic <t>mastocytoma</t> <t>P815-GFP</t> (K d D d ) at a ratio of 2:1. Nontransduced (NTR) T cells were used as the control. Cytotoxic activity of transduced T cells was analyzed by flow cytometry after 24 h of culturing by evaluating the percentage of dead P815 tumor cells as GFP + PI + cells. Data from 2 to 4 independent experiments are shown as mean ± SD. * P < 0.05, ** P < 0.01 versus the NTR control, unpaired Student’s t test.
    P815 Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p815-gfp/pm15336644-283-0-17
    Average 90 stars, based on 1 article reviews
    p815-gfp - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

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    T cells transduced with the dominant-active α-chain TCRs killed cognate tumor cells in vitro. T cells of intact C57BL/6 mice (H-2 b ) were modified with identified dominant-active TCRα from the repertoire of primarily activated effectors (EF) or reactivated memory T cells (EM) (Figs. and ). Transduced T cells were mixed with the allogeneic mastocytoma P815-GFP (K d D d ) at a ratio of 2:1. Nontransduced (NTR) T cells were used as the control. Cytotoxic activity of transduced T cells was analyzed by flow cytometry after 24 h of culturing by evaluating the percentage of dead P815 tumor cells as GFP + PI + cells. Data from 2 to 4 independent experiments are shown as mean ± SD. * P < 0.05, ** P < 0.01 versus the NTR control, unpaired Student’s t test.

    Journal: Computational and Structural Biotechnology Journal

    Article Title: The Abundance of α-Chain-Centric TCRs in the Mouse Repertoire of Primarily Activated Effectors and Reactivated Memory T Cells

    doi: 10.34133/csbj.0026

    Figure Lengend Snippet: T cells transduced with the dominant-active α-chain TCRs killed cognate tumor cells in vitro. T cells of intact C57BL/6 mice (H-2 b ) were modified with identified dominant-active TCRα from the repertoire of primarily activated effectors (EF) or reactivated memory T cells (EM) (Figs. and ). Transduced T cells were mixed with the allogeneic mastocytoma P815-GFP (K d D d ) at a ratio of 2:1. Nontransduced (NTR) T cells were used as the control. Cytotoxic activity of transduced T cells was analyzed by flow cytometry after 24 h of culturing by evaluating the percentage of dead P815 tumor cells as GFP + PI + cells. Data from 2 to 4 independent experiments are shown as mean ± SD. * P < 0.05, ** P < 0.01 versus the NTR control, unpaired Student’s t test.

    Article Snippet: Murine mastocytoma P815 (K d D d ) [TIB-64, American Type Culture Collection (ATCC)], P815-GFP, lymphoma EL4 (K b D b ) (TIB-39, ATCC), and EL4-GFP were obtained from the collection of N.N.

    Techniques: Transduction, In Vitro, Modification, Control, Activity Assay, Flow Cytometry